Journal: Scientific Reports

Article Title: Cognitive deficits and anxiety induced by diisononyl phthalate in mice and the neuroprotective effects of melatonin

doi: 10.1038/srep14676

Figure Lengend Snippet: The coronal sections were cut at Bregma-3.8 mm. Representative images of the expression of ( A ) Caspase-3 in the CA 1 region; ( B ) Caspase-3 in the cerebral cortex; ( C ) GFAP in the CA 1 region; ( D ) GFAP in the cerebral cortex. Analyses of caspase-3 and GFAP expression levels according to average optical density.

Article Snippet: Rabbit anti-caspase-3-antibody, rabbit anti-GFAP- antibody, goat-anti-rabbit lgG-antibody, rabbit lgG peroxidase conjugated streptavidin-biotin complex (SABC-POD) kit and a diaminobenzidine (DAB) kit were obtained from Boster Bio-engineering (Boster Bio-engineering, Wuhan, China).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Cognitive deficits and anxiety induced by diisononyl phthalate in mice and the neuroprotective effects of melatonin

doi: 10.1038/srep14676

Figure Lengend Snippet: The coronal sections were cut at Bregma-3.8 mm. Representative images of the expression of ( A ) Caspase-3 in the CA 1 region; ( B ) Caspase-3 in the cerebral cortex; ( C ) GFAP in the CA 1 region; ( D ) GFAP in the cerebral cortex. Analyses of caspase-3 and GFAP expression levels according to average optical density.

Article Snippet: Rabbit anti-caspase-3-antibody, rabbit anti-GFAP- antibody, goat-anti-rabbit lgG-antibody, rabbit lgG peroxidase conjugated streptavidin-biotin complex (SABC-POD) kit and a diaminobenzidine (DAB) kit were obtained from Boster Bio-engineering (Boster Bio-engineering, Wuhan, China).

Techniques: Expressing

Journal: PLoS ONE

Article Title: T-Helper Type-2 Contact Hypersensitivity of Balb/c Mice Aggravated by Dibutyl Phthalate via Long-Term Dermal Exposure

doi: 10.1371/journal.pone.0087887

Figure Lengend Snippet: Representative images of the expression of (A) IL-5 and (B) IL-13 as determined by immunohistochemical staining (brown color stain) (black arrow). Panel: (A1, B1) Saline, (A2, B2) DBP 0.4, (A3, B3) DBP 4, (A4, B4) DBP 40, (A5, B5) FITC, (A6, B6) FITC+DBP 0.4, (A7, B7) FITC+DBP 4, (A8, B8) FITC+DBP 40. Analyses of (C) IL-5 and (D) IL-13 expression levels according to average optical density. Animal groups (in all panels): n = 4 mice per group. **: p <0.01, compared with saline group; ##: p <0.01, compared with FITC group.

Article Snippet: Mouse anti-IL-5-antibody, mouse anti-IL-13-antibody, and mouse anti-eosinophil cationic protein (ECP)-antibody, goat-anti-rabbit lgG-antibody, a rabbit lgG peroxidase conjugated streptavidin-biotin complex (SABC-POD) kit and a diaminobenzidine (DAB) kit were obtained from Boster Bio-engineering (Wuhan, China).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of intranasal administration with Bacillus subtilis on immune cells in the nasal mucosa and tonsils of piglets

doi: 10.3892/etm.2018.6093

Figure Lengend Snippet: Information of antibodies.

Article Snippet: 2 , Goat anti-pig IgA , A100-102P , 1:200 , Bethyl Laboratories, Inc., Montgomery, TX, USA , SABC-POD (goat IgG) Kit , SA1023 , 1:1 , Boster Biological Technology.

Techniques:

Journal: Molecular Therapy. Nucleic Acids

Article Title: MicroRNA-29a-3p Reduces TNFα-Induced Endothelial Dysfunction by Targeting Tumor Necrosis Factor Receptor 1

doi: 10.1016/j.omtn.2019.10.014

Figure Lengend Snippet: TNF-R1 Expression Is Repressed by miR-29a-3p (A) Schematic representation of the 3′ UTR of TNFRSF1A shows a putative miR-29a-3p binding site. The seed location for the WT 3′ UTR of TNFRSF1A is indicated in red, whereas the Mut 3′ UTR is indicated in blue. (B) The effect of miR-29a-3p on the 3′ UTR of TNFRSF1A was determined by luciferase activity assays. HEK293 cells were cotransfected with TNFSF1A -3′ UTR wild-type (WT) or mutant (Mut) plasmid, and miR-29a-3p or its inhibitor by Lipofectamine 2000. After 24 h, luciferase activities were measured. (C–F) TNF-R1 protein expression was measured in EA.hy926 cells (C), HAECs (D), pHUVECs (E), and HUVSMCs (F) that had been differentially transfected. A scrambled sequence was used as a negative control (NC). After 48 h of transfection, TNF-R1 protein expression was assessed by western blot. Each test was performed at least three times. Error bars are defined as the SD. *p < 0.05 and **p < 0.01 indicate statistically significant differences. miR-29a-3p, cells transfected with miR-29a-3p mimic.

Article Snippet: Subsequently, the sections were incubated with different primary antibodies for 48 h. Immunohistochemistry for TNF-R1 was performed with a mouse anti-TNF-R1 primary antibody diluted 1:50 (Santa Cruz Biotechnology, CA, USA) and a SABC-POD Mouse IgG kit (Boster Biological Technology, China).

Techniques: Expressing, Binding Assay, Luciferase, Activity Assay, Mutagenesis, Plasmid Preparation, Transfection, Sequencing, Negative Control, Western Blot

Journal: Molecular Therapy. Nucleic Acids

Article Title: MicroRNA-29a-3p Reduces TNFα-Induced Endothelial Dysfunction by Targeting Tumor Necrosis Factor Receptor 1

doi: 10.1016/j.omtn.2019.10.014

Figure Lengend Snippet: miR-29a-3p Affects TNFα-Induced VCAM-1, ICAM-1, and E-selectin Expression by Targeting TNF-R1 (A–C) The mRNA levels of VCAM-1 (A), ICAM-1 (B), and E-selectin (C) were detected in pHUVECs transfected with miR-29a-3p or/and the plasmid containing human TNFRSF1A (TNF-R1 overexpression). (D–F) The mRNA levels of VCAM-1 (D), ICAM-1 (E), and E-selectin (F) were measured in pHUVECs transfected with miR-29a-3p inhibitor or/and TNF-R1-siRNA. After 24 h, the cells were treated with TNFα for 3 h before collection. A scrambled sequence was used as a negative control (NC). Each test was performed three times. Error bars are defined as the SD. *p < 0.05 and **p < 0.01 indicate statistically significant differences. miR-29a-3p, cells transfected with miR-29a-3p mimic.

Article Snippet: Subsequently, the sections were incubated with different primary antibodies for 48 h. Immunohistochemistry for TNF-R1 was performed with a mouse anti-TNF-R1 primary antibody diluted 1:50 (Santa Cruz Biotechnology, CA, USA) and a SABC-POD Mouse IgG kit (Boster Biological Technology, China).

Techniques: Expressing, Transfection, Plasmid Preparation, Over Expression, Sequencing, Negative Control

Journal: Molecular Therapy. Nucleic Acids

Article Title: MicroRNA-29a-3p Reduces TNFα-Induced Endothelial Dysfunction by Targeting Tumor Necrosis Factor Receptor 1

doi: 10.1016/j.omtn.2019.10.014

Figure Lengend Snippet: miR-29a-3p Agomir Affects TNF-R1 Expression in Arteries In Vivo (A) Schematic representation of experimental mouse design is shown. Briefly, 8-week-old male mice received a tail vein injection of saline or miR-29a-3p agomir and were then administered an intraperitoneal injection of saline or TNFα. (B) Immunohistochemistry staining was performed for TNF-R1 in the Mouse aorta at harvest. Error bars are defined as the SD. Scale bars, 50 μm. **p < 0.01 indicates statistically significant differences.

Article Snippet: Subsequently, the sections were incubated with different primary antibodies for 48 h. Immunohistochemistry for TNF-R1 was performed with a mouse anti-TNF-R1 primary antibody diluted 1:50 (Santa Cruz Biotechnology, CA, USA) and a SABC-POD Mouse IgG kit (Boster Biological Technology, China).

Techniques: Expressing, In Vivo, Injection, Saline, Immunohistochemistry, Staining

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on VEGFR2 protein expression in rat vascular endothelial cells. ( A ) Western blot bands of indicating proteins in endothelial cells; ( B ) densitometric analysis of the ratio of the intensity of protein bands. Values are the mean ± SD of VEGFR2 expression standardized to β-actin expression in three independent experiments; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on proliferation and migration of endothelial cells. ( A ) BrdU-positive cell rate; ( B ) migration distance statistics chart. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Migration

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on tube formation of endothelial cells. ( A ) Number of nodes forming a tube-like structure; ( B ) branch lengths forming the tube-like structure. Data expressed as mean ± SD; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques:

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the mouse matrigel plug model. ( A ) Pictures of the mouse matrigel plugs (a: PBS group, b: NC group, c: ROX group, d: sh-Vegfr2 group, and e: sh-Vegfr2 + ROX group); ( B ) hemoglobin content in matrigel plugs. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; # p < 0.05, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques:

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the growth and angiogenesis of/in melanoma xenografts. ( A ) Melanoma xenograft tumor volumes during the test period; ( B ) melanoma xenograft tumor weight at necropsy; ( C ) immunohistochemical expression of CD34 in melanoma xenografts. Data expressed as mean ± SD; * p < 0.05, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Immunohistochemical staining, Expressing